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ISSN : 1226-9999(Print)
ISSN : 2287-7851(Online)
Korean Journal of Environmental Biology Vol.35 No.4 pp.622-630

Isolation, Regeneration and the Maturation Induction of Free-Living Gametophytes of Undaria pinnatifida (Phaeophyceae)

Hyun Il Yoo,Su Hong Kim,Gi hyun Lee,Dong Soo Ha,Eun Kyoung Hwang


The induction of regeneration and the maturation of the free-living gametophytes of Undaria pinnatifida were studied at temperatures (5, 10, 15 and 20℃), irradiance (5, 10, 20 and 40 μmol photons m-2 s-1) and photoperiods (14 : 10, 12 : 12 and 10 : 14 h L : D). Female gametophyte fragments were maintained in active regeneration without them reaching sexual maturity under conditions of 15℃, 20 μmol photons m-2 s-1, 10 : 14 h (L : D); by contrast, the conditions for male gametophytes were slightly different at 15℃, 5 μmol photons m-2 s-1, 14 : 10 h (L : D). The sexual maturation of female and male gametophytes took place under 5℃, 20-40 μmol photons m-2 s-1, 10 : 14 h (L : D) and 15℃, 40 μmol photons m-2 s-1, 10 : 14 h (L : D), respectively. These results provide basic information for controlling the regeneration and maturation of free-living gametophytes for srain improvement as well as the cross breeding of U. pinnatifida.

갈조류 미역 유리배우체의 분리, 재생 및 성 숙 유도